Journal: Cell and tissue research

Article Title: Prolonged high fat diet ingestion, obesity, and type 2 diabetes symptoms correlate with phenotypic plasticity in myenteric neurons and nerve damage in the mouse duodenum

doi: 10.1007/s00441-015-2132-9

Figure Lengend Snippet: Antibody manufacturer and dilutions used for immunohistochemistry

Article Snippet: All dilution information for primary antibodies, secondary antibodies and dyes can be found in . table ft1 table-wrap mode="anchored" t5 caption a7 Primary Antibody, Dilution Secondary Antibody, Dilution Dye, Dilution All Neurons Ms monoclonal HuC/HuD, 1:200 Molecular Probes, Eugene, OR {"type":"entrez-nucleotide","attrs":{"text":"A21271","term_id":"514139","term_text":"A21271"}} A21271 Dk anti Ms IgG Dylight 488, 1:300 Jackson mmunoResearch, West Grove, PA 715-485-150 N/A Inhibitory Motor Neurons Rb polyclonal VIP, 1:1000 Abcam, Cambridge, MA Ab43841 Biotinylated Gt anti Rb IgG, 1:300 Vector Labs, Burlingame, CA BA1000 Streptavidin Alexa Fluor 488 conjugate, 1:600; Jackson ImmunoResearch, West Grove, PA 016-540-084 Rb polyclonal nNOS, 1:500 Invitrogen, Camarillo, CA #61-7000 Biotinylated Gt anti Rb IgG, 1:300 Vector Labs, Burlingame, CA BA1000 Streptavidin Cy3 conjugate, 1:600 Life Technologies, Grand Island, NY SA1010 Sheep polyclonal nNOS, 1:500 Abcam, Cambridge, MA Ab6175 Dk anti Sheep IgG Dylight 594, 1:300 Jackson ImmunoResearch, West Grove, PA 715-485-150 N/A Excitatory Motor Neurons Gt polyclonal ChAT, 1:200 Millipore, Billerica, MA AB144P Biotinylated Rb anti Gt IgG, 1:300 Vector Labs, Burlingame, CA BA5000 N/A Rt monoclonal Substance P, 1:150 Millipore, Billerica, MA MAB356 Biotinylated Gt anti Rt, 1:300 Invitrogen, Camarillo, CA #62–9540 Streptavidin Cy3 conjugate, 1:600 Life Technologies, Grand Island, NY SA1010 Sensory Neurons Rabbit polyclonal anti-Calbindin D-28k, 1:1250 CH-1723, Marly 1 (Switzerland) CB 38 Biotinylated Gt anti Rb IgG, 1:300 Vector Labs, Burlingame, CA BA1000 Streptavidin Cy3 conjugate, 1:600 Life Technologies, Grand Island, NY SA1010 Rb polyclonal CGRP, 1:200 Sigma Aldrich, St. Louis, MO # c8198 Biotinylated Gt anti Rb IgG, 1:300 Vector Labs, Burlingame, CA BA1000 Streptavidin Cy3 conjugate, 1:600 Life Technologies, Grand Island, NY SA1010 Apoptosis Rb polyclonal anti-cleaved Caspase-3, Cell Signaling Technology (ASP 175) Dk anti rabbit IgG Dylight 488, 1:300 N/A Open in a separate window Ms: Mouse, Dk: Donkey, Rb: Rabbit, Gt: Goat, Rt: Rat Antibody manufacturer and dilutions used for immunohistochemistry To ensure the specificity of staining, co-localization of nucleated neuronal cells was confirmed through DAPI counterstaining, and primary and secondary antibody negative control staining was performed for all antibodies used.

Techniques: Plasmid Preparation